|Institut de Pharmacologie Moléculaire et Cellulaire|
mining microRNAs targets across large scale gene expression studies
MiRonTop is an online java web tool that integrates DNA microarrays or high-throughput sequencing data to identify the potential implication of miRNAs on a specific biological system. It allows a rapid characterization of the most pertinent mRNA targets according to several existing miRNA target prediction approaches. It also provides useful representations of the enrichment scores according to the position of the target site along the 3’-UTR, where the contribution of the sites located in the vicinity of the stop codon and of the polyA tail can be clearly highlighted. It provides different graphs of miRNA enrichment associated with up- or down-regulated transcripts and different summary tables about selections of mRNA targets and their functional annotations by Gene Ontology.
MiRopTop will then explore the top table and identify for each miRNA considered, an enrichment of its predicted targets according to the selected prediction tool accross the DOWN and the UP gene sets, significance being evaluated using the hypergeometric law.
If you make use of the data presented here, please cite the following article:
MiRonTop: mining microRNAs targets across large scale gene expression studies.
Le Brigand K, Robbe-Sermesant K, Mari B, Barbry P.
Bioinformatics, 2010 Oct 19.