Computation of high throughput sequencing coverage

Required coverage or reads for Illumina sequencing on NextSeq500

Genome resequencing / de novo	30X
Whole Exome sequencing	100X
Custom capture enrichment	100X
ChIP-seq	30M (T.Factor), 50M (Histone marks)
RNA-seq	30M (polyA), 50M (ribozero)
smallRNA-seq	20M reads

Sequencing type	Genome Exome custom capture chIP-seq RNA-seq smallRNA-seq
Fragment type	Fragment Mate-paired Paired-end
Size of fragment	75 150 200 400
Number of libraries (1 per mate-paired lane)
Length of targeted region in Mb (genome, exome or capture)	Mb.
Number of reads (NextSEQ run is 400M or 130M)	Millions reads

Bases / read	150 b.
Bases total generated	60 Gb.
Bases total / library	5 Gb.
Reads total / library	33.3 Millions    ok
Coverage / library	100 X